Sterilization of cshake flasksell

Cell shaker is a triangular cell culture container, mostly used for suspension cell culture or medium mixing and preparation.

Cell shaker is a triangular cell culture container, mostly used for suspension cell culture or medium mixing and preparation.

Sterility is essential for cell culture experiments, so by what means is this consumable sterilized?

At present, we have a lot of common sterilization methods, cell shaker is mainly cobalt 60 sterilization method, it is the use of 60Co γ-ray irradiation, the role of microorganisms, direct or indirect destruction of microbial cell nuclei, thereby killing microorganisms, play a role in sterilization.

It is one of the irradiation sterilization technology, the radiation isotope cobalt 60 produces γ- rays on the product irradiation treatment. In the process of energy transfer and transfer, produce powerful physical and biological effects, to achieve the purpose of insecticide, sterilization, inhibition of physiological processes.

60Co - γ-ray irradiation sterilization is a special "cold processing" technology, it is sterilized at room temperature, γ-ray high energy, strong penetration, no pollution of the environment, no residue, sterilization effect is complete, in the sterilization at the same time, will not cause the internal temperature of the cell shaker to rise, and therefore is called "cold" sterilization method.

Generally, the sterilized cell shaker will be stored in vacuum packaging and opened before use.

It is important to note that although sterilized, we must still strictly observe aseptic practices when using Cell shaker to avoid introducing exogenous contamination.